| 1. | A murine phage antibody library containing 1 . 710
成功构建一库容量为1 . 710 |
| 2. | Then the biotin - labelled n - peptide interacted with the phage antibody library
对其研究可能有助于了解成瘾机制和有临床应用价值。 |
| 3. | Construction of murine phage antibody library and selection of n - peptide - binding single - chain antibodies
小鼠噬菌体抗体库的构建和n -肽结合单链抗体的筛选 |
| 4. | Selection of anti - hcg scfv from murine phage antibody library selection of anti - hcg scfv from murine phage antibody library
从噬菌体抗体库中淘选人绒毛膜促性腺激素的单链抗体 |
| 5. | The murine phage antibody library was amplified and then panned by human chorionic gonadotropin for four rounds . the last round enriched phage clones were used to reinfect
由鼠源噬菌体抗体库淘选到展示有人绒毛膜促性腺激素单链抗体的噬菌体克隆。 |
| 6. | One n - peptide - binding scfv clone was selected from the phage antibody library using affinity panning . the target antigen , n - peptide was biotinylated using photobiotin first
N -肽是一种新发现的神经肽，其n端与阿片肽高度同源，可与阿片肽受体相互作用。 |
| 7. | In the present paper the application of dna library , phage display random peptide library , phage antibody library , etc . in the research of serodiagnostic reagents and their special values were reviewed
本文综述了基因文库、噬菌体展示肽库及噬菌体抗体库技术在血清学诊断试剂研制中的应用及其各自的优点。 |
| 8. | The patterns of sds - page indicated more than 30 % of recombinant proteins could be obtained from the extract of e . coli bl21 . furthermore , library of recombinant phage antibodies was constructed from total rna isolated from spleen of the female balb / c mice immunized by bull sperm
首先用牛精子免疫雌性四周龄balb c小鼠，从其脾脏组织分离总rna ，应用重组噬菌体抗体库技术，构建了一个针对牛精子的噬菌体抗体文库。 |
| 9. | The library was rescued with phage m13k07 in order to display scfv on the surface of the phage and to form the recombinant phage antibody library . one of positive scfv clones , named pcsal , was selected with phage - elisa after panning and screening by bull sperm three times . scfv fragment , amplified from pcsa1 , was ligated to pmd18 - t vector for sequencing analysis
取阳性重组噬菌体抗体克隆株pcsa1 ， pcr扩增其scfv基因，筛选重组子进行序列测定，发现其序列符合小鼠抗体基因的一般特征，并且与几株抗磷酸胆碱的抗体重链和轻链可变区序列的同源性达80以上；推测pcsa1scfv针对的抗原是磷酸胆碱类物质。 |
| 10. | Methods : a set of oligonucleotide primers were designed and used to amplify the vh and vl gene from anti - hbsag fab antibodies screened from phage antibody library . the products were cloned into puc19 vector and their sequences were analysed . the vh and vl gene fragments were tethered by a peptide linker and a leader sequence coding region , with the leader sequence added at 5 " terminus of each gene ( l - vh - linker - vl ) and designated as l - scfv
方法以从噬菌体抗体库中筛选获得的抗hbsag的fab抗体基因为模板，分别扩增出其轻、重链可变区( v _ l 、 v _ h )基因，通过重组pcr方法将轻、重链可变区基因用连接肽( gly _ 4ser ) _ 3的编码序列连接，并引入前导肽编码序列，构建具有l - v _ h - linker - v _ l结构的单链抗体基因。 |
